Coding
BisdAB

Part:BBa_K525515:Design

Designed by: Anna Drong   Group: iGEM11_Bielefeld-Germany   (2011-09-10)

Fusion protein of BisdA and BisdB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 603
    Illegal BamHI site found at 1341
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 1594
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Fusion protein of BisdA and BisdB in Freiburg BioBrick assembly standard (RFC 25).


Source

  • bisdA and bisdB originated in Sphingomonas bisphenolicum AO1


References

Sasaki M, Tsuchido T, Matsumura Y (2008) Molecular cloning and characterization of cytochrome P450 and ferredoxin genes involved in bisphenol A degradation in Sphingomonas bisphenolicum strain AO1, [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2008.03843.x/full J Appl Microbiol 105(4):1158-1169].